Hemoglobinopathies arc the most common genetic disorder and constitute an increasing global burden. Incidence is highest in Middle East and Indian subcontinent as per WHO. Hemoglobinopathies can be screened using a single. highly excellent me1hod with the use of High-Performance Liquid Chromatography. AIM: The present study was carried out to assess spectrum of hemoglobinopathies using HPLC method in our hospital. MATERIALS AND METHODS: Retrospective study from July 2019 to August 202 1 was done which included total 610 cases,. CBC parameters and peripheral smears were compared with the chromatographs in all patients. RESULTS: Our study shows prevalence of~ thalassemia trait in females in the age group of 2 I to 40 years. One of the cases showed compound heterozygous for HbS and thalassemia. CONCLUSIONS: HPLC is rapid accurate and useful method for diagnosing hemoglobinopathies in developing countries like India where resources are limited.