Materials & Methods: The study on insertion of brachialis muscle was performed on 100 (200 specimens of superior extremities) embalmed donated cadavers (90 males & 10 females) in the department of Anatomy of K.J.Somaiya Medical College, Sion, Mumbai, India. The dissection of the arm and forearm was done meticulously to expose the brachialis muscle. The insertion of brachialis muscle was observed. The neuro vascular pattern of the upper limb were also observed. The photographs of the insertion of brachialis muscles were taken for proper documentation. Observations: Out of 200 specimens of superior extremities the insertion of brachialis muscle in to the bicipital aponeurosis was observed in 4 specimens. In all the 4 specimens along with the biceps brachii muscle, the brachialis got inserted in to the bicipital aponeurosis. The bicipital aponeurosis was merged with the deep fascia of fore arm which got merged with the aponeurosis attached to the posterior subcutaneous border of the ulna. The median nerve and the brachial artery travelled deep to this additional insertion of brachialis muscle in to the bicipital aponeurosis. The associated altered anatomy of the nerves and vessels were not observed. All the variations were unilateral. Conclusion: The knowledge of such type of variation is clinically important for Anatomists, Physiotherapists, Neurologists, Radiologists, Surgeons, Plastic Surgeons and Orthopedicians.