Aim to study two unusual separate biceps brachii muscle. 100 upper limbs of 50 donated embalmed cadavers (45 males & 5 females) of age group ranging from 70 to 80 years were dissected in the department of Anatomy at K. J. Somaiya Medical College, Sion, Mumbai, INDIA. The two unusual separate biceps brachii muscle supplied by the Median nerve were observed in 2 specimens. The vascular pattern in the arm was also observed. The photographs of the variation of the formation of median nerve were taken for proper documentation. The unusual separate short and long heads of the biceps brachii were observed in 2 specimens. The short head originated from the tip of the coracoid process of the scapula. The long head originated from the supraglenoid tubercle of the scapula. The short head of the biceps brachii muscle got inserted into the radial tuberosity of the radius separately. The long head got inserted into the radial tuberosity and bicipital aponeurosis. The brachial artery and the median nerve ran in between the two heads throughout their course in the arm up to the cubital fossa. The musculocutaneous nerve was absent and all the muscles of the front of the arm were supplied by the median nerve. There was no associated arterial variation seen in the specimen. Topographical anatomy and variations of the bicephs brachii muscle is clainically important for surgeons, orthopaedicians, radiologists and anaesthetists performing pain anagement therapies.