Candida is a yeast-like fungus and omnipresent in the environment, which makes it one of the most common pathogens for opportunistic infections. Candida usually causes mucosal Candidiasis but may also cause systemic and disseminated infections in debilitated patients. Phospholipase production has been reported to be one of the virulence factors in Candida. This study was carried out to isolate various species of Candida from different clinical specimens and to check their Phospholipase activity. All the clinical specimens suspected of fungal aetiology were analysed and subjected to Microbiological identification of Candida species. Candida species were subjected toPhospholipase activity by Egg Yolk Agar Plate method. A total of 7 varieties of Candida species ( C.albicans 25.45%, C. tropicalis 32.72%, C. krusei 12.72%, C. kefyr 12.72%, C. dubliniensis 10.90%, C. glabrata 3.63%,C.utilis 1.81%) were isolated from various clinical samples.Out of total 54 isolates, 29(53.70%)showed extensive Phospholipase activity.